Restriction digests of the clone give the following sizes (kb): BamHI--7.8; EcoRI--5.0, 2.4; SacI/XbaI--3.8, 3.2, 0.42.
High copy number shuttle expression vector.
this vector has the TEF promoter for expression, the LEU2 marker gene and the 2 micron replicon. It is distributed in E. coli.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504
