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hE61.f1
hE61.f1
規(guī)格:
價格:
編號:B186091
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
hE61.f1
商品貨號
B186091
Designations
hE61.f1
GenBank Number

U18991

培養(yǎng)基
Species
Homo sapiens, human
Depositors
DA Thompson
Vector
Construct size (kb): 5.599999904632568
Insert
DNA: cDNA
Insert lengths(kb): 2.599999904632568
Tissue: retinal pigment epithelium
Gene product: retinal pigment epithelium-specific protein (65kD) [RPE65]
Insert Size (kb)
2.600
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 2.7; SmaI--4.4, 1.0; PstI--2.9, 1.8, 0.65.
The 2.6kb insert differs from the GenBank entry as follows: A17C, G35C, G36A, G234A, G2573C, T2601C and it contains 24 additional nucleotides at the 3' end.
The 24 additional nucleotides (GGG CTG CAG GAA TTC CTG CAG GGG) introduce EcoRI, SmaI, PstI restriction sites at the 3' end of the insert.
Inserts detects an insertion/deletion polymorphism at the following nucleotides of the GenBank record G2384del and A2385del.
References

Nicoletti A, et al. Molecular characterization of the human gene encoding abundant 61 kDA protein specific to the retinal pigment epithelium. Hum. Mol. Genet. 4: 641-649, 1995. PubMed: 7633413

Gu SM, et al. Mutations in RPE65 cause autosomal recessive childhood-onset severe retinal dystrophy. Nat. Genet. 17: 194-197, 1997. PubMed: 9326941

Debra A Thompson, personal communication

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