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pE25.a [pBS300]
pE25.a [pBS300]
規(guī)格:
價格:
編號:B182196
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pE25.a [pBS300]
商品貨號
B182196
Designations
pE25.a [pBS300]
Depositors
IE Scheffler
培養(yǎng)基
Vector
Vector: pE25.a
Size: 3,200  bp 
Selectable Marker:  ampicillin resistant
Cloning sites: EcoR I, Sac I, Kpn I, Sma I, BamHI, Xba I, Sal I, Pst I, Sph I, Hind III
Replicon: pUC (pMB1)
Features:  Lac, T3 and T7 promoters
Insert
DNA: genomic
Insert lengths(kb): 0.349
Tissue: DNA from hamster/human XJM12.1.3 hybrid cell line
Gene product: DNA Segment, repetitive [D1Z7]
Insert Size (kb)
0.349
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Plasmid in Escherichia coli
Comments
The insert contains the following sites (in bp from an end): Sau3A (20), HaeIII(52), PstI (85), HinfI (181), HaeIII (227).
Restriction digests of the clone give the following sizes (kb): EcoRI--3.2, 0.35; BamHI--3.6; HindIII--3.6; KpnI--3.6; HincII--3.6.
The first half of the dimer is 85% identical to the sequence of the 1' monomer; the second half is 80% identical to the 2' monomer.
The insert is a dimer of the 171 bp consensus sequence of alphoid DNA from the subfamily of an alpha-satellite DNA made up of EcoRI dimers or multiples thereof.
References

Carine K, et al. Molecular characterization of human minichromosomes with centromere from chromosome 1 in human-hamster hybrid cells. Somatic Cell Mol. Genet. 15: 445-460, 1989. PubMed: 2781415

Immo E Scheffler, personal communication

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