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pKA-1
pKA-1
規格:
價格:
編號:B178896
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pKA-1
商品貨號
B178896
Designations
pKA-1
GenBank Number

U07562

培養基
Species
Homo sapiens, human
Depositors
S Clifton, BA Roe
Vector
Construct size (kb): 19.10000038146973
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC
Intact vector size: 2.700
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites:
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1
terminator: none
Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ABL1
Other gene symbols: ABL
Genomic copy number: unique
Gene name: Abelson murine leukemia viral (v-abl) oncogene homolog 1
Contains complete coding sequence?: N
Chromosome: 9; Localization: 9 q34.1
Tissue: chronic myelogenous leukemia K-562 cell line
Type of DNA: genomic
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 16.421
Cross references: DNA Seq. Acc.: U07562
Nucleotides 1-16321 of the insert correspond to
nucleotides 42692-59012 of U07562.
Insert lengths(kb): 16.42099952697754
Tissue: chronic myelogenous leukemia K-562 cell line
Gene product: Abelson murine leukemia viral (v-abl) oncogene homolog 1 [ABL]
Alleles: D1, D2, D1, D2, A1, A2, B2, C1, C2, A1, A2, B1, B2, C1, C2, B1
Insert Size (kb)
16.421
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--9.2, 6.4, 2.3, 2.1; BglI--7.2, 5.5, 2.5, 2.2, 1.3, 1.2; HindIII--11.5, 7.6, 0.82; PstI--5.1, 4.8, 3.75, 2.7, 2.1, 0.73, 0.64; EcoRI--7.0, 5.0, 2.65, 2.15, 1.1, 0.92, 0.38.
The insert contains part of the intron between exon 1b and exon 1a.
References

Grosveld G, et al. The chronic myelocytic cell line K562 contains a breakpoint in bcr and produces a chimeric bcr/c-abl transcript. Mol. Cell. Biol. 6: 607-609, 1986. PubMed: 3023859

Chissoe SL, et al. Sequence and analysis of the human ABL gene, the BCR gene, and regions involved in the Philadelphia chromosomal translocation. Genomics 27: 67-82, 1995. PubMed: 7665185

Bruce A Roe, personal communication

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