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pTK-HSV-BP2 plasmid in <em>Escherichia coli</em>

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編號:B177845

品牌:Mingzhoubio

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RNA

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產品名稱

pTK-HSV-BP2 plasmid in Escherichia coli

商品貨號

B177845

Designations

pTK-HSV-BP2 plasmid in Escherichia coli

GenBank Number

U02031

培養基

Species

Homo sapiens, human

Depositors

JL Goldstein

Applications

in suitable host, produces protein sterol regulatory element binding transcription factor 2 sterol regulatory element-binding protein 2

Vector

Name of vector: pTK-HSV
Intact vector size: 5.8
Type of vector: phagemid
Vector end: BspDI
Vector end: XbaI
Cloning sites: BspDI XbaI
Construction: pcDNA3
Host range: mammalian cells; Escherichia coli

Features (with orientation and position when available):
replicon: pMB1
marker(s): neoR, ←
replicon: f1, ←
terminator: bGH polyadenylation
restriction site: XbaI
restriction site: BspDI
epitope tag: HSV
promoter: HSV TK, ←
marker(s): ampR, →

Insert

Insert size (kb): 4.2
Gene: sterol regulatory element binding transcription factor 2, SREBP2
DNA: cDNA
Source: human
Genbank accession : U02031
Nucleotides: Nucleotides 1-4093 of the insert correspond to nucleotides 156-4249 of U02031

Insert Size (kb)

4.2

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Freeze dried E. coli containing the plasmid

Comments

Restriction digests of the clone give the following sizes (kb): BspDI/XbaI--5.8, 4.2; EcoRI--6.8, 3.0; HindIII--9.0; 0.8.

The insert contains the following restriction sites (approximate kb from the 5' end): EcoRV--1.08; HindIII--1.12; BglII--1.70; EcoRI--2.45

This construct allows the exogenously expressed SREBF2 to be cleaved in a sterol-regulated fashion after transfection into 293 cells (ATCC CRL-1573).

The amino terminus was truncated at amino acid 14 and fused to the HSV tag of the vector.

References

Hua X, et al. Hairpin orientation of sterol regulatory element-binding protein-2 in cell membranes as determined by protease protection. J. Biol. Chem. 270: 29422-29427, 1995. PubMed: 7493979

Joseph L Goldstein, personal communication

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