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當前位置: 首頁 > ATCC代理 > pKR1B-LAC4-1 [L1582/pKR1B-LAC4-1, YNN27/pKR1B-LAC4-1] B177117
pKR1B-LAC4-1 [L1582/pKR1B-LAC4-1, YNN27/pKR1B-LAC4-1]
pKR1B-LAC4-1 [L1582/pKR1B-LAC4-1, YNN27/pKR1B-LAC4-1]
規格:
價格:
編號:B177117
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pKR1B-LAC4-1 [L1582/pKR1B-LAC4-1, YNN27/pKR1B-LAC4-1]
商品貨號
B177117
Designations
pKR1B-LAC4-1 [L1582/pKR1B-LAC4-1, YNN27/pKR1B-LAC4-1]
GenBank Definition

Yeast (K.lactis) beta-galactosidase gene, 5' end

培養基
GenBank Number

M18108

Species
Kluyveromyces lactis (Dombrowski) van der Walt
Depositors
University of Kentucky, RC Dickson, University of Kentucky
Applications
produces protein beta-galactosidase beta-D-galactosidase
produces protein lactose permease
This is a Lac+ strain expressing K. lactis lactose permease and beta-galactosidase.
Vector
Construct size (kb): 20.0
Insert
DNA: genomic
Insert lengths(kb): 12.0
Gene product: beta-galactosidase(beta-D-galactosidase) [LAC4]
Target Gene: lactose permease, beta-galactosidase
Insert Size (kb)
12.0
Genotype
MATalpha trp1-289 ura3-52 gal2
Media
ATCC® Medium 1245: YEPD
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
The plasmid contains the following restriction sites (approximate kb from the pBR322-derived EcoRI site): XhoI--2.5; EcoRI--8.1, 9.1, 11.4, 11.9, 12.6, 15.1, 16; BamHI--14.6, 19.7.
Fermentation of lactose in whey
GenBank M18108 contains the sequence for the promoter region and part of the coding sequence for LAC4.
The vector is a derivative of pBR322 carrying the kanamycin (G418) resistance gene of Tn903 and ARS1B from Kluyveromyces lactis. This plasmid can replicate in E. coli, S. cerevisiae, and K. lactis.
The order of the major features in this plasmid is: ampR - kanR - LAC12 - LAC4 > - ARS1B.
References

Sreekrishna K, et al. Transformation of Kluyveromyces lactis with the kanamycin (G418) resistance gene of Tn903. Gene 28: 73-81, 1984. PubMed: 6329916

Sreekrishna K, Dickson RC. Construction of strains of Saccharomyces cerevisiae that grow on lactose. Proc. Natl. Acad. Sci. USA 82: 7909-7913, 1985. PubMed: 3934664

Leonardo JM, et al. Identification of upstream activator sequences that regulate induction of the beta-galactosidase gene in Kluyveromyces lactis. Mol. Cell. Biol. 7: 4369-4376, 1987. PubMed: 3125422

Dickson RC, Sreekrishna KK. Lac+ Saccharomyces cerevisiae. US Patent 5,047,340 dated Sep 10 1991

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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