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HHCPS21
HHCPS21
規(guī)格:
價(jià)格:
編號(hào):B172996
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
HHCPS21
商品貨號(hào)
B172996
Designations
HHCPS21
GenBank Number

M78220

培養(yǎng)基
Species
Homo sapiens, human
Vector
Construct size (kb): 7.400000095367432
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: IRF1
Gene name: interferon regulatory factor 1
Contains complete coding sequence?: U
Chromosome: 5; Localization: 5 q23-q31
Tissue: hippocampus, female, 2 years, oligo-dT and random primed
(Stratagene 936205), subtracted
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 4.4
Cross references: DNA Seq. Acc.: M78220; EST: EST01813; Primer: M13 Forward;
THC id: THC78075
Insert lengths(kb): 4.400000095367432
Tissue: hippocampus, female, 2 years, oligo-dT and random primed (Stratagene 936205), subtracted
Gene product: interferon regulatory factor 1 [IRF1]
Insert Size (kb)
4.400
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRi--2.95, 1.65, 1.4 (doublet); PstI--4.4, 2.9.
EST01813 matches GenBank accession HUMIRF1 with 98.28% identity over the sequence range: 313-139.
References

Boultwood J, et al. Allelic Loss of IRF1 in myelodysplasia and acute myeloid leukemia: retention of IRF1 on the 5q-chromosome in some patients with the 5q-syndrome. Blood 82: 2611-2616, 1993. PubMed: 8219215

Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749

J C Venter, personal communication

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