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pLF108
pLF108
規(guī)格:
價(jià)格:
編號(hào):B172911
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
pLF108
商品貨號(hào)
B172911
Designations
pLF108
GenBank Number

M11147

培養(yǎng)基
Species
Homo sapiens, human
Depositors
JW Drysdale
Vector
Construct size (kb): 5.21999979019165
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: PstI
Vector end: PstI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: FTL
Genomic copy number: unique
Gene name: ferritin, light polypeptide
Contains complete coding sequence?: Y
Chromosome: 19; Localization: 19 q13.3-q13.4
Tissue: liver
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 0.823
Cross references: DNA Seq. Acc.: M11147
Insert lengths(kb): 0.8230000138282776
Tissue: liver
Gene product: ferritin, light polypeptide [FTL]
Insert Size (kb)
0.823
Media
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): SacI--5.0; PvuII--2.8, 2.1; NotI--5.0; HIndIII--4.5, 0.3, 0.25; PstI--4.3, 0.65, 0.25.
A PstI digest excises the insert as two fragments (bp): 593, 230. The smaller fragment contains the 5' untranslated region.
The insert contains the following restriction sites (bp from the 5' end): HindIII--569, 809; NcoI--450; NotI--372; PstI--224; PvuII--405; SacI--154.
Contains the complete coding sequence (nt 152-679).
References

Boyd D, et al. Structural and functional relationships of human ferritin H and L chains deduced from cDNA clones. J. Biol. Chem. 260: 11755-11761, 1985. PubMed: 3840162

Jim W Drysdale, personal communication

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