Restriction digests of the clone give the following sizes (kb): HindIII--4.4; PstI--4.4.
Plasmid vector for bacterial expression of nonfusion proteins and protein domains.
The synthetic DNA used in construction contains translational start and stop signals and unique restriction sites. The galK marker is expressed from lambda PL.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Sczakiel G, et al. An efficient expression vector (pPLEX) for bacterial systhesis of non-fusion proteins and protein domains. Nucleic Acids Res. 15: 1878, 1987. PubMed: 3029730