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Nup475
Nup475
規(guī)格:
價(jià)格:
編號(hào):B172569
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Nup475
商品貨號(hào)
B172569
Designations
Nup475
GenBank Number

J05669

培養(yǎng)基
Species
Mus musculus, mouse
Depositors
D Nathans
Vector
Construct size (kb): 4.684000015258789
Insert
DNA: cDNA
Insert lengths(kb): 1.814000010490418
Tissue: 3T3 cell line
Gene product: Nup475 protein (immediate early nuclear protein)( NUP475, tristetraprolin, zinc finger protein homologous to Zfp-36 in mouse) [Nup475]
Insert Size (kb)
1.814
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--3.7, 0.7, 0.4; BglI--2.7, 1.8, 0.35; EcoRI--3.0, 1.8; PvuII--4.7; SacI--3.3, 1.4.
The insert contains the following restriction sites (kb from the 5' end): BglI--0.199, 0.508; SacI--0.338; BamHI--0.854, 1.195.
Detects mouse genomic restriction fragments of the following sizes (kb): EcoRI--8.8; HindIII--7.1. Simple banding patterns were also detected with human and rat DNA.
Contains the complete coding sequence and 3' untranslated region and is missing 16 bp from the 5' untranslated region. The ORF of 959 nt begins at nt 60 of the complete mRNA.
The insert is oriented such that transcription from the SP6 promoter gives the sense strand.
Classification
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

DuBois RN, et al. A growth-factor inducible nuclear protein with a novel cysteine/histidine repetitive sequence. J. Biol. Chem. 265: 19185-19191, 1990. PubMed: 1699942

Daniel Nathans, personal communication

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