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pLinkCAT

規格:

價格:

編號:B172555

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pLinkCAT

商品貨號

B172555

Designations

pLinkCAT

Depositors

PL Davies

培養基

Biosafety Level

Vector Information

Size (kb): 4.5000000000000000

Vector: pLinkCAT (plasmid)

Promoters: Promoter none

Construction: pSV2cat, polylinker

Marker(s):ampR

Construct size (kb): 4.5

Features: insert detection: CAT
marker(s): ampR
promoter: none
replicon: pMB1
terminator: none
enhancer: none

Applications

promoter-cloning vector

shuttle vector

Comments

Restriction digests of the clone give the following sizes (kb): HindIII--4.5; BglII--4.5; SstI--4.5; consistent with deposited DNA.

This is a promoter-cloning shuttle plasmid vector with a polylinker which can facilitate both molecular cloning of a DNA fragment as well as controlled deletion of sequence from either end of the insert.

This was constructed from the large AccI/HindIII pSV2CAT fragment and a polylinker with AccI and HindIII ends such that the AccI at the end was lost.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Wu YL, et al. A modified CAT expression vector with convenient cloning sites. Nucleic Acids Res. 18: 1919, 1990. PubMed: 2336378

Shipped

freeze-dried

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