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HHCME18

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編號:B172520

品牌:Mingzhoubio

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DNA

RNA

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產品名稱

HHCME18

商品貨號

B172520

Designations

HHCME18

GenBank Number

M78011

培養基

Species

Homo sapiens, human

Vector

Construct size (kb): 4.699999809265137

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:

Insert

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ENO1
Genomic copy number: unique
Gene name: enolase 1, (alpha)
Contains complete coding sequence?: U
Chromosome: 1; Localization: 1 p36
Tissue: hippocampus, female, 2 years, oligo-dT and random-primed
(Stratagene 936205)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.7
Cross references: DNA Seq. Acc.: M78011; EST: EST01596; Primer: M13 Forward;
THC id: THC123491
DNA Seq. Acc.: M86157; EST: EST02688; Primer: M13 Reverse;
THC id: THC123491

Insert lengths(kb): 1.700000047683716

Tissue: hippocampus, female, 2 years, oligo-dT and random-primed (Stratagene 936205)

Gene product: enolase 1, (alpha)(phosphopyruvate hydratase) [ENO1]

Insert Size (kb)

1.700

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.7; BamHI--4.7; PstI--4.7; uncut.

EST01596 matches GenBank accession HUMENOAL4 with 95.06% identity over the sequence range: 386-1.

EST02688 matches GenBank accession HUMENOA with 98.84% identity over the sequence range: 1-346.

References

Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749

J C Venter, personal communication

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