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pSVBV-E3
pSVBV-E3
規(guī)格:
價格:
編號:B172433
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pSVBV-E3
商品貨號
B172433
Designations
pSVBV-E3
Species
strawberry vein banding caulimovirus
培養(yǎng)基
Depositors
TJ Morris
Applications
Non-infectious full-length clone of strawberry vein banding virus useful in diagnosing the infection.
Vector
Construct size (kb): 10.5
Insert
DNA: genomic
Insert lengths(kb): 7.800000190734863
Insert Size (kb)
7.800
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
(BamHI-290-) HindIII-1050- XbaI-875- HindIII-100- AvaI-125- XhoI-570- XbaI-190- HindIII-100- XbaI-100- HindIII-190- EcoRI. The PstI, KpnI and BamHI sites are unique within the insert.
Insert contains the following sites separated by (bp)(approx): EcoRI-475- XbaI-140- HindIII-1050- HindIII-170- EcoRV-50- NcoI-70- AvaI-100- XhoI-190- PstI-570- HindIII-190- NcoI-190- HindIII-620- EcoRV-100- AvaI-50- KpnI-30- EcoRV-290- BamHI-290-.
Restriction digests of the clone give the following sizes (kb): PstI--9.4, 2.4; BamHI--6.8, 5.1; EcoRI--8.6, 3.0.
Distribution and titer of virion DNA varied greatly between leaflets from the same infected plant.
Non-infectious full-length clone of strawberry vein banding virus useful in diagnosing the infection.
Isolated from Fragaria vesca. Purified virion DNA digested with EcoRI and cloned into EcoRI site of pUC8. Digested recombinants that co-migrated with full-length linear virion DNA were further screened by Southern blot.
Classification
Caulimovirus
References

Stenger DC, et al. Isolation, molecular cloning, and detection of strawberry vein banding virus DNA. Phytopathology 78: 154-159, 1988.

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