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Dr-1
Dr-1
規(guī)格:
價格:
編號:B172234
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Dr-1
商品貨號
B172234
Designations
Dr-1
GenBank Number

M97388

培養(yǎng)基
Species
Homo sapiens, human
Vector
Construct size (kb): 4.349999904632568
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: DR1*
Genomic copy number: unique
Gene name: transcription repressor (interacting with the TATA-binding
protein)
Contains complete coding sequence?: Y
Tissue: HeLa cell line
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.4
Cross references: DNA Seq. Acc.: M97388
Insert lengths(kb): 1.399999976158142
Tissue: HeLa cell line
Gene product: transcription repressor (interacting with the TATA-binding protein) [DR1*]
Insert Size (kb)
1.400
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.9, 0.74; KpnI--3.3, 1.4; SstI--4.6; PvuII--2.6, 0.9, 0.72, 0.58; XbaI--3.2, 0.94, 0.58; BamHI/HindIII--2.9, 1.70.
The insert contains the following restriction sites (approximate kb from the 5' end): EcoRI--0.68; KpnI--0.14, 0.15; SmaI--0.44; PvuII--0.40, 1.09; XbaI--0.32, 1.2.
Detects transcripts of 3.4 and 1.5 kb.
Transcription from the T7 promoter gives the sense strand.
References

Inostroza JA, et al. Drl, a TATA-binding protein-associated phosphoprotein and inhibitor of class II gene transcription. Cell 70: 477-489, 1992. PubMed: 1339312

Juan A Inostroza, personal communication

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