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pESD 14.1.1 [pESD14.1]

規(guī)格:

價(jià)格:

編號(hào):B172144

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

pESD 14.1.1 [pESD14.1]

商品貨號(hào)

B172144

Designations

pESD 14.1.1 [pESD14.1]

Species

Homo sapiens, human

培養(yǎng)基

Depositors

RA Phillips

Vector

Construct size (kb): 4.300000190734863

Insert

DNA: cDNA

Insert lengths(kb): 1.200000047683716

Tissue: SV40-transformed fibroblast

Gene product: esterase D/formylglutathione hydrolase( S-formylglutathione hydrolase, esterase D) [ESD]

Alleles: A1, A2

Insert Size (kb)

1.200

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): XhoI/PstI--1.8, 1.2, 1.1, 0.15; PstI--3.1, 1.28; XhoI--3.0, 1.35; BamHI--3.1, 0.65; HindIII--4.3.

To obtain a full length (1.2 kb) fragment of the cDNA, cut with XhoI. To remove all vector sequences but 40 bp at the 3' end, cut with XhoI/PstI.

The ApaI RFLP is distinct from the allelic isozymes because of different allele frequencies and because all 4 haplotypes of the 2 polymorphisms have been detected.

References

Squire J, et al. Cloning of the esterase D gene: A polymorphic gene closely linked to the retinoblastoma locus on chromosome 13. Proc. Natl. Acad. Sci. USA 83: 6573-6577, 1986. PubMed: 3462714

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