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GnRHR
GnRHR
規格:
價格:
編號:B172134
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
GnRHR
商品貨號
B172134
Designations
GnRHR
GenBank Number

L03380

培養基
Species
Homo sapiens, human
Depositors
SS Kakar
Applications
in another host, produces protein gonadotropin-releasing hormone receptor
Vector
Construct size (kb): 5.559999942779541
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pcDNA I
Intact vector size: 4.000
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: HindIII BamHI BstXI EcoRI PstI EcoRV NotI XhoI SphI NsiI
XbaI
Polylinker sites: HindIII BamHI BstXI EcoRI PstI EcoRV BstXI NotI XhoI SphI
NsiI XbaI
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
enhancer: CMV
promoter: CMV, ->
promoter: T7, ->
MCS: HindIII...XbaI, ->
promoter: SP6, <-
terminator: SV40 polyadenylation, ->
replicon: polyoma
replicon: SV40
replicon: pMB1
replicon: M13, <-
marker(s): supF, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GRHR
Genomic copy number: unique
Gene name: gonadotropin-releasing hormone receptor
Contains complete coding sequence?: Y
Tissue: pituitary
Type of DNA: cDNA
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 1.56
Cross references: DNA Seq. Acc.: L03380
Insert lengths(kb): 1.559999942779541
Tissue: pituitary
Gene product: gonadotropin-releasing hormone receptor [GRHR]
Target Gene: gonadotropin-releasing hormone receptor
Insert Size (kb)
1.560
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digest of the clone give the following sizes (kb): EcoRI--4.0, 1.55; BamHI--4.95, 0.6; HindIII--4.3, 0.8, 0.4; PstI--4.65, 0.9; PvuII--2.2, 1.8, 1.55.
The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.55; HindIII--0.42, 1.26; NdeI--1.06; NcoI--0.53; PstI--0.65; PvuII--0.70; SphI--1.42.
Contains the complete coding sequence (nt 56-1042).
The following primers can be used to amplify an 800 bp DNA fragment contained in the gene: upstream 5'-GCTTGAAGCTCTGTCCTGGGA-3'; downstream 5'-CCTAGGACATAGTAGGG-3'.
When transfected into COS-7 cells, expresses a high-affinity receptor.
Detects a 5.0 kb RNA transcript in human pituitary.
Transcription from the T7 promoter gives the sense strand.
References

Kakar SS, et al. Cloning, sequencing, and expression of human gonadotropin releasing hormone (GnRH) receptor. Biochem. Biophys. Res. Commun. 189: 289-295, 1992. PubMed: 1333190

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