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p423 CYC1
p423 CYC1
規格:
價格:
編號:B171941
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
p423 CYC1
商品貨號
B171941
Designations
p423 CYC1
Depositors
M Funk
培養基
Biosafety Level
1
Vector Information
Size (kb): 6.3159999847412110
Vector: p423 CYC1 (plasmid)
Promoters: Promoter for expression CYC1
Construction: pRS423
Marker(s):ampR,HIS3
Construct size (kb): 6.315999984741211
Features: marker(s): HIS3
marker(s): ampR
promoter for expression: CYC1
replicon: 2 micron
MCS: SalI...XbaI
terminator: CYC1
Applications
YX-type (expression) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.2, 2.8, 0.35; XbaI--3.4, 2.9; HindIII--4.8, 1.4.
Contains a truncated version of the CYC1 promoter that very weak and no longer inducible because of a deletion of most of the UAS2 sequence.
High copy number shuttle expression vector.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

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component of:ATCC 87669
Shipped
freeze-dried
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