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pMOB4
pMOB4
規(guī)格:
價(jià)格:
編號(hào):B171836
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pMOB4
商品貨號(hào)
B171836
Designations
pMOB4
Species
pCU1
培養(yǎng)基
Depositors
HP Schweizer
Vector
Construct size (kb): 5.800000190734863
Insert
DNA: genomic
Insert lengths(kb): 2.700000047683716
Gene product: (IncN) origin of transfer replication [oriT]
Insert Size (kb)
2.700
Media
ATCC Medium 1065 (see below) plus kanamycin (50.0 mcg/ml) plus chloramphenicol (25.0 mcg/ml) ATCC Medium 1065: Tryptone (Difco 0123), 10.0 g Yeast Extract (Difco 0127), 5.0 g NaCl, 10.0 g Distilled water, 1.0 L
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): XbaI--5.8; NotI--3.4, 2.4; EcoRI/HindIII--2.8, 1.5, 0.55 (doublet), 0.2.
Available for research purposes only.
Plasmid containing an oriT/cmlR mobilization cassette that can be inserted into a general purpose vector (ATCC 77278, 77279) for conjugal transfer of cloned inserts into the chromosome of P. aeruginosa.
Constructed by deleting the 2.5 kb XbaI fragment from pMOB3 (ATCC 77282). The NotI digest excises a fragment of approximately 3.0 kb that includes short segments of the pHSS21 sequence.
The NotI cassette includes these features: pHSS21 sequence - EcoRI - oriT - PstI - BamHI - HindIII - cmlR - HindIII - BamHI - XbaI - sacR fragment/SalI - PstI - SphI - HindIII - pHSS21 sequence.
References

Schweizer HP. Allelic exchange in Pseudomonas aeruginosa using novel ColE1-type vectors and a family of cassettes containing a portable oriT and the counter-selectable Bacillus subtilis sacB marker. Mol. Microbiol. 6: 1195-1204, 1992. PubMed: 1588818

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