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CD28Ig
CD28Ig
規格:
價格:
編號:B171812
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
CD28Ig
商品貨號
B171812
Designations
CD28Ig
GenBank Number

J02988

M27286

培養基
Species
Homo sapiens, human
Depositors
Bristol-Myers Squibb Co., P Linsley, Bristol-Myers Squibb Co.
Applications
in another host, produces protein CD28 antigen - IgCgamma1 chimera
Vector
Construct size (kb): 5.199999809265137
Insert
DNA: cDNA
Insert lengths(kb): 0.4300000071525574
Tissue: T-cell tumor cell line HPB-ALL
Gene product: immunoglobulin gamma 1 (Gm marker)(immunoglobulin heavy chain constant region, gamma 1) [CD28]
Target Gene: CD28 antigen - IgCgamma1 chimera
Insert Size (kb)
0.430
Media
ATCC® Medium 1890: LB medium (ATCC medium 1065) with 12 mcg/ml tetracycline and 100 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--5.2; HindIII/XbaI--4.2, 1.2; PstI--5.2; XbaI--5.2; XhoI--5.2.
The Ig hinge cysteines were mutated to serine residues during construction to prevent intrachain disulfide bonding.
Expresses a soluble derivative of CD28, consisting of the oncostatin M signal sequence, the extracellular portion of CD28, and the hinge, CH2 and CH3 portions of Ig Cgamma1.
References

Linsley PS. Chimeric CTLA4 receptor and methods for its use. US Patent 5,434,131 dated Jul 18 1995

Aruffo A, Seed B. Molecular cloning of a CD28 cDNA by a high-efficiency COS cell expression system. Proc. Natl. Acad. Sci. USA 84: 8573-8577, 1987. PubMed: 2825196

Linsley PS, et al. Binding of the B cell activation antigen B7 to CD28 costimulates T cell proliferation and interleukin 2 mRNA accumulation. J. Exp. Med. 173: 721-730, 1991. PubMed: 1847722

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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