黄色在线视频播放,精品不卡一区二区三区,青娱乐青青草

lambdagt11 phage

規(guī)格:

價格:

編號:B171804

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

加入購物車

在線咨詢收藏

產(chǎn)品名稱

lambdagt11 phage

商品貨號

B171804

Designations

lambdagt11 phage

Depositors

R Young

培養(yǎng)基

Biosafety Level

Vector Information

Size (kb): 43.70

Vector: lambdagt11 (phage, lambda - replacement)

Promoters: Promoter lac

Construction: lambdagt7-lac5(b522 nin5); lambdagt4(cI857 S100 nin5)

Construct size (kb): 43.70

Features: insert detection: lacZ
promoter: lac
replicon: lambda
repressor gene: cI857

Applications

expression vector

vector for constructing cDNA libraries

vector permitting construction of fusion proteins

Comments

The EcoRI site divides the vector into a left arm of 19.6 kb and a right arm of 24.1 kb.

lambdagt11 is an expression vector that permits insertion of foreign DNA into lacZ and promotes regulated synthesis of hybrid proteins.

Growth Conditions

Temperature: 30.0°C

References

Young RA, Davis RW. Efficient isolation of genes by using antibody probes. Proc. Natl. Acad. Sci. USA 80: 1194-1198, 1983. PubMed: 6219389

Young RA, Davis RW. Yeast RNA polymerase II genes: isolation with antibody probes. Science 222: 778-782, 1983. PubMed: 6356359

Huynh TV, et alConstructing and screening cDNA libraries in lambdagt10 and lambdagt11In: Huynh TV, et alDNA cloning techniques: a practical approach1OxfordIRL Presspp. 49-78, 1985

Sambrook J, et al. Molecular cloning: a laboratory manual. 1Cold Spring Harbor, NY: CSHL Press; 1989.