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HHCPR13

規(guī)格:

價(jià)格:

編號(hào):B171794

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

HHCPR13

商品貨號(hào)

B171794

Designations

HHCPR13

GenBank Number

M78212

培養(yǎng)基

Species

Homo sapiens, human

Vector

Construct size (kb): 4.0

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:

Insert

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ATP1A2
Genomic copy number: unique
Gene name: ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide
Contains complete coding sequence?: U
Chromosome: 1; Localization: 1 q21-q23
Tissue: hippocampus, female, 2 years, oligo-dT and random primed
(Stratagene 936205), subtracted
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 0.7
Cross references: DNA Seq. Acc.: M78212; EST: EST01805; Primer: M13 Forward;
THC id: THC122363
DNA Seq. Acc.: M86176; EST: EST02708; Primer: M13 Reverse;
THC id: THC122363

Insert lengths(kb): 0.699999988079071

Tissue: hippocampus, female, 2 years, oligo-dT and random primed (Stratagene 936205), subtracted

Gene product: ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide [ATP1A2]

Insert Size (kb)

0.700

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--3.3, 0.7; BamHI--4.0; PvuII--2.8, 1.2.

EST01805 matches GenBank accession HUMATP1A2 with 99.64% identity over the sequence range: 1-278.

EST02708 contains an Alu repeat element.

References

Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749

J C Venter, personal communication

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