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pH52 [PH52, ph52]

規格:

價格:

編號:B171764

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pH52 [PH52, ph52]

商品貨號

B171764

Designations

pH52 [PH52, ph52]

GenBank Number

M24747

培養基

Species

Homo sapiens, human

Vector

Construct size (kb): 4.28000020980835

Insert

DNA: cDNA

Insert lengths(kb): 1.080999970436096

Tissue: teratocarcinoma

Gene product: tumor rejection antigen (gp96) 1(tumor rejection antigen gp96) [TRA1]

Insert Size (kb)

1.081

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--5.6, 1.0; HindIII--6.6; EcoRI/HindIII--3.0, 2.6, 1.0; BglI--2.4, 1.8, 1.2, 0.6 + smaller; PstI--3.0, 1.9, 1.5.

The following enzymes do not cut within the insert: AatI, AatII, AvaI, BglII, EcoRI, EcoRV, HaeII, HindIII, PvuII, SalI, SstI, XbaI, XhoI.

The insert contains the following restriction sites (nucleotide position according to the published sequence): BamHI--30; BglI--109; KpnI--599, PstI--1038.

This clone has a 2.6 kb EcoRI/HindIII fragment of unknown origin. Our EcoRI digest is consistent with deposited DNA.

The insert extends from nucleotide 13 to 1094 of the published sequence. The coding sequence extends from 106 to 2517, with the signal peptide encoded from 106 to 168.

References

Maki RG, et al. Human homologue of murine tumor rejection antigen gp96: 5'-regulatory and coding regions and relationship to stress-induced proteins. Proc. Natl. Acad. Sci. USA 87: 5658-5662, 1990. PubMed: 2377606

Pramod K Srivastava, personal communication

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