IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Cosmid vector which facilitates restriction enzyme mapping.
Derived from phage lambda origin of replication.
Media
ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 30.0°C
References
Little PF, Cross SH. A cosmid vector that facilitates restriction enzyme mapping. Proc. Natl. Acad. Sci. USA 82: 3159-3163, 1985. PubMed: 2987911
