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MES-OV
MES-OV
規(guī)格:
價(jià)格:
編號(hào):B171427
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
MES-OV
商品貨號(hào)
B171427
Organism
Homo sapiens, human
Tissue
ovary
培養(yǎng)基
Product Format
frozen 1.0 mL
Morphology
epithelial-like
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
ovarian serous cystadenocarcinoma, stage III, grade 2
Age
53 years
Gender
female
Applications
This is an excellent in vitro and in vivo model of ovarian cancer. Useful for gene expression profiling. RefSchaner ME, et al. Gene expression patterns in ovarian carcinomas. Mol. Biol. Cell 14: 4376-4386, 2003. PubMed: 12960427
Storage Conditions
liquid nitrogen vapor phase
Images
Cell Micrograph of MES-OV Cells, ATCC CRL-3272
Derivation
CRL-3272 cells were isolated from the ascites fluid from a 53 year-old female patient with stage III ovarian cystadenocarcinoma.
Clinical Data
53 years old
female
Cellular Products
collagen, lumican, matrix metalloproteinase 2, SPARC (secreted protein acidic and rich in cysteine), AXL receptor tyrosine kinase
Comments
CRL-3272 cells were isolated from the ascites fluid from a 53 year-old female patient with Stage III ovarian cystadenocarcinoma. Clonogenic assays performed at the time of isolation indicated that this cell line is quite sensitive to vinca alkaloids (vinblastine) (Deposit Form Information). Based on gene expression profiling, MES-OV manifests some mesenchymal features, with high expression of collagens (type VI, alpha 3, type I, alpha 2, type III, and alpha 1), lumican, matrix metalloproteinase 2, and SPARC. This cell line lacks many of the distinct molecular characteristics of epithelial cells, typically seen in ovarian neoplasms, although expression of MUC1 and mesothelin was notable (PubMed: 12960427). This metastatic cell line also highly expresses AXL receptor tyrosine kinase protein (PubMed: 20858715).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) (ATCC 30-2200) or 0.05% (w/v) Trypsin - 0.53 mM EDTA (ATCC® PCS-999-003) solution to remove all traces of serum which contains trypsin inhibitor.
  2. Add 2.0 to 3.0 ml of 0.05% (w/v) Trypsin - 0.53 mM EDTA (ATCC® PCS-999-003) solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

   Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  1. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
    1. Add appropriate aliquots of the cell suspension to new culture vessels.  An inoculum of 8 X 103 to 4 X 104 viable cells/cm2 is recommended.
    2. Incubate cultures at 37°C. Subculture when cell density reaches between 1 X 105 and 2 X 105 cells/cm2

 

Subcultivation Ratio: 1:4 to 1:10 is recommended.

Medium Renewal: 2 to 3 times a week

Cryopreservation
Freeze Medium: Complete growth medium, 90%; DMSO, 10%
Storage Temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Volume
1.0 mL
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 9,11
D16S539: 11,12
D5S818: 13
D7S820: 11
TH01: 6,9
TPOX: 8,11
vWA: 17
Population Doubling Time
approximately 22 hours (personal communication)
Name of Depositor
B Sikic, Stanford University
Year of Origin
1980
References

Wang YC, et al. Regional activation of chromosomal arm 7q with and without gene amplification in taxane-selected human ovarian cancer cell lines. Genes Chromosomes Cancer 45: 365-374, 2006. PubMed: 16382445

Rankin EB, et al. AXL is an essential factor and therapeutic target for metastatic ovarian cancer. Cancer Res. 70(19): 7570-7579, 2010. PubMed: 20858715

Schaner ME, et al. Gene expression patterns in ovarian carcinomas. Mol. Biol. Cell 14: 4376-4386, 2003. PubMed: 12960427

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