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Preceptrol®

no

Genotype

F- supC(Ts) lac(Am) mal trp(Am) rpsL htpR165 pho(Am) lambda-

Type Strain

no

Comments

The depositor indicated that this strain was derived from CAG-456. The genotype is not complete because the nature of the reversion is not known.

This strain has reduced proteolysis of foreign proteins. It does not require heat induction to reduce proteolysis and has the potential to accumulate foreign protein over a longer period of time.

Medium

ATCC^® Medium 1065: LB Agar/Broth, Miller

Growth Conditions

Temperature: 30.0°C

Name of Depositor

W Mandecki

References

Mandecki W, et al. High-level expression of a gene encoding the human complement facator C5a in Escherichia coli. Gene 43: 131-138, 1986. PubMed: 3530879

Baker TA, et al. A gene regulating the heat shock response in Escherichia coli also affects proteolysis. Proc. Natl. Acad. Sci. USA 81: 6779-6783, 1984. PubMed: 6387713