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Preceptrol®

no

Genotype

F- supE44 hsdR17 mcrA+ mcrB+ endA1 thi-1 aroB mal- cI857 lambdaPR tetR lambda-

Type Strain

no

Comments

This host is useful for vectors with PR promoters so that expression is regulated by the cI857 repressor. Such vectors include pEX11 (ATCC 37708), pEX12 (ATCC 37709) and pEX13 (ATCC 37710).

Constructed by cloning a 2.3 kb BglII fragment containing the cI857 and PR promoter into pOM41 and then transferring into the chromosome of C600. This was introduced into the MM294 background by P1 transduction with aroB.

Medium

ATCC^® Medium 1065: LB Agar/Broth, Miller

Growth Conditions

Temperature: 37.0°C

Name of Depositor

BA Van Der Zeijst

Chain of Custody

ATCC <<--BA Van Der Zeijst<<--O. Raibaud

References

Kusters JG, et al. Improvement of the cloning linker of the bacterial expression vector pEX. Nucleic Acids Res. 17: 8007, 1989. PubMed: 2678010