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RT4-D6P2T
RT4-D6P2T
規(guī)格:
價(jià)格:
編號(hào):B167209
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
RT4-D6P2T
商品貨號(hào)
B167209
Organism
Rattus norvegicus, rat
Tissue
peripheral nervous system
培養(yǎng)基
Cell Type
neuronal Schwann cell
Product Format
frozen
Morphology
neuronal
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Schwannoma
Applications
This cell line is a suitable transfection host.
Storage Conditions
liquid nitrogen vapor phase
Images
Derivation
RT4-D6P2T is an schwannoma cell line derived from a N-ethyl-N-nitrosourea (ENU) induced rat peripheral neurotumor, RT4. The tumor was grown in culture and a cell line RT4-AC was isolated that subsequently developed in a distinct cell type, RT4-D that produces glial specific S100. RT4-D was subcloned to produce RT4-D6 that was subcloned to produce RT-4-D6P2 and then finally subcloned to produce the D6P2T subclone. RefBansal R, Pfeiffer SE. Regulated galactolipid synthesis and cell surface expression in Schwann cell line D6P2T. J. Neurochem. 49: 1902-1911, 1987. PubMed: 2824698
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping, do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by pipetting gently.
  5. Add appropriate aliquots of cell suspension to new culture vessels. An split ratio of 1:5 to 1:10 is recommended.
  6. Incubate culture vessels at 37°C.


Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells: a Manual of Basic Technique by R. Ian Freshney, 3th edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Name of Depositor
PI Patel
References

Hai M, et al. Comparative analysis of Schwann cell lines as model systems for myelin gene transcription studies. J. Neurosci. Res. 69: 497-508, 2002. PubMed: 12210843

Bansal R, Pfeiffer SE. Regulated galactolipid synthesis and cell surface expression in Schwann cell line D6P2T. J. Neurochem. 49: 1902-1911, 1987. PubMed: 2824698

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