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Mino
Mino
規格:
價格:
編號:B167208
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Mino
商品貨號
B167208
Organism
Homo sapiens, human
Tissue
peripheral blood/Mantle cell lymphoma (B cell non-Hodgkin's lymphoma)
培養基
Cell Type
lymphoblast; cytogenetic abnormalit RefLai R, et al. Establishment and characterization of a new mantle cell lymphoma cell line, Mino. Leuk. Res. 26: 849-855, 2002. PubMed: 12127561
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Mantle Cell Lymphoma
Age
64
Gender
male
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor phase
Karyotype
Modal chromosome number: 74. This is a hypertriploid male cell line, del(6)(q16), t(8;11)(q22;q23), add(11)(q25), add(13)(q34), add(14)(q32). Most cells contain the t(11;14) derivative chromosome, detected by FISH with whole chromosome paint probes.
Images
Clinical Data
64
Caucasian
male
Antigen Expression
CD3-, CD5+, CD10-, CD19+, CD20+, CD23- (verified at ATCC)
Oncogene
p53, p16 (INK4a), and p21(WAF1)
Genes Expressed
cyclin D2 and cyclin D3, p53, p16 (INK4a), and p21(WAF1)
Comments
The cells are large, growing singly and in small clumps in vitro. The immunophenotype by flow cytometry is compatible with MCL. Western blots show expression of cyclin D1 but no detectable cyclin D2 and cyclin D3; the retinoblastoma protein is predominantly phosphorylated. There is expression of tumor suppressor gene products including p53, p16 (INK4a), and p21(WAF1).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
Subculturing

Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 6 X 104 viable cells/mL. Do not allow the cell concentration to reach 1.5 X 106 cells/mL.

Subcultivation Ratio: 1:5 to 1:10

Cryopreservation
90% FBS; 10% (v/v) DMSO
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X,Y
CSF1PO: 9,11
D13S317: 12
D16S539: 11,12
D5S818: 11,12
D7S820: 10,11
THO1: 9.3
TPOX: 8,11
vWA: 14,17
Population Doubling Time
about 72 hours
Name of Depositor
R.J.Ford
References

Lai R, et al. Establishment and characterization of a new mantle cell lymphoma cell line, Mino. Leuk. Res. 26: 849-855, 2002. PubMed: 12127561

Marzec M, et al. Mantle cell lymphoma cells express predominantly cyclin D1a isoform and are highly sensitive to selective inhibition of CDK4 kinase activity. Blood 108: 1744-1750, 2006. PubMed: 16690963

Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365

Lai R, et al. Establishment and characterization of a new mantle cell lymphoma cell line, Mino. Leuk. Res. 26: 849-855, 2002. PubMed: 12127561

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