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VMM5A
VMM5A
規格:
價格:
編號:B167207
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
VMM5A
商品貨號
B167207
Organism
Homo sapiens, human
Tissue
Melanoma, Lymph Node Metastasis
培養基
Cell Type
Melanocyte
Product Format
frozen
Morphology
Epithelial-like
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Melanoma, Stage IIIC; malignant
Age
81
Gender
Male
Ethnicity
Caucasian
Applications
Drug screening
Development of targeted therapy
Development of combination therapy
Tumor vaccine development
Storage Conditions
liquid nitrogen vapor phase
Images
ATCC CRL-3226 Cell Micrograph
Derivation
Derived from tumors taken from tumor-involved lymph nodes from patients at the University of Virginia
Clinical Data
Primary Site: Right Posterior Shoulder; Metastatic Site: Lymph Node, Right Neck
Antigen Expression
Positives: High VEGF-R2, Low GP100, Tyrosinase, Low MAGE-A1, MMP-1
HLA Typing
A2.1,A1101,B39,B44, BW4,BW6,C7(17)DR7, DR8,DR11,DQ2,DQ7
Comments
NRAS: wt
CDKN2A: wt
BRAF Mutation: V600E
APC Mutation
PDGFRA Mutation: D842V
PTEN Mutation: R173H 
more sensitive to BAY43-9006 (BRAF kinase inhibitor) and to
rapamycin (mTOR kinase inhibitor), compared to cell lines with wild-type B-Raf

Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing

Volumes are for a T-75 flask; Adjust accordingly

  1. Remove and discard the cell culture medium from the flask.
  2. Rinse the cell monolayer with Dulbecco’s PBS without calcium or magnesium and remove.
  3. Add 3 to 4 ml of the trypsin-EDTA solution, rotate flask to rinse cell monolayer, remove trypsin solution, and incubate at 37oC.
  4. Once the cells appear to be detached, add 10 ml of complete growth medium with a pipette to the cell suspension to inactivate the trypsin. Gently wash any remaining cells from the growth surface of the   flask. Check the  cells with the microscope to be sure that most (>95%) are single cells. If cell clusters are apparent, continue to disperse the cells with gentle pipetting.
  5. Subculture as necessary.
  6. Place the flask back into the incubator. Examine the culture the following day to ensure the cells have reattached and are actively growing.
  7. Repeat when cells reach confluence.
Cryopreservation
Fetal bovine serum, 90%; DMSO, 10%
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2 ), 5%
STR Profile
Amelogenin: X,Y
D5S818: 9
D7S820: 9,10
D13S317: 12
D16S539: 12
vWA: 16
TH01: 9.3
TPOX: 8,11
CSF1PO: 11
Sterility Tests

Pass

Name of Depositor
Craig L. Slingluff, Jr. M.D.
Passage History
Unknown. 2 passages from 2003 frozen cell line stock, but it is not known how long the line was in culture after being established from tumor tissue obtained in 1992.
Year of Origin
1992
References

Slingluff C, et al. Melanomas with concordant loss of multiple melanocytic differentiation proteins: immune escape that may be overcome by targeting unique or undefined antigens. Cancer Immunol. Immunother. 48:661-672, 2000. PubMed: 10752474

Molhoek K, et al. Human melanoma cytolysis by combined inhibition of mammalian target of rapamycin and Vascular Endothelial Growth Factor/Vascular Endothelial Growth Factor Receptor-2. Cancer Res. 68: (11), 2008. PubMed: 18519701

Molhoek K, et al. Comprehensive analysis of RTK activation in human melanomas reveals autocrine signaling through IGF-1R. Melanoma Res 21(4): 274–284, 2011. PubMed: 21654344

Molhoek K, et al. Synergistic inhibition of human melanoma proliferation by combination treatment with B-Raf inhibitor BAY43-9006 and mTOR inhibitor Rapamycin. J. Trans. Med. 3(39): 2005. PubMed:16255777

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