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當(dāng)前位置: 首頁(yè) > ATCC代理 > P3/NSI/1-Ag4-1 [NS-1] B167202
P3/NSI/1-Ag4-1 [NS-1]
P3/NSI/1-Ag4-1 [NS-1]
規(guī)格:
價(jià)格:
編號(hào):B167202
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
P3/NSI/1-Ag4-1 [NS-1]
商品貨號(hào)
B167202
Organism
Mus musculus, mouse
Cell Type
B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
plasmacytoma; myeloma
Strain
BALB/c
Applications
Kappa chains are synthesized but not secreted.
Tested and found negative for ectromelia virus (mousepox).
The cells are resistant to 0.1 mM 8-azaguanine and do not grow in HAT medium.
Storage Conditions
liqid nitrogen vapor phase
Images
Derivation
This is a non-secreting clone of P3X63Ag8 (ATCC TIB-9).
Antigen Expression
Antigen expression: H-2d
Genes Expressed
immunoglobulin (not secreted),H-2d
Cellular Products
immunoglobulin (not secreted)
Comments
Kappa chains are synthesized but not secreted.
The cells are resistant to 0.1 mM 8-azaguanine and do not grow in HAT medium.
The cells have been reported to be cholesterol auxotrophs due to a deficiency in 3-ketosteroid reductase activity.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 105 viable cells/mL. Maintain cell density between 1 X 105 and 1 X 106 viable cells/mL. Do not allow the cell density to exceed 1 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liqid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Isotype
kappa light chain
Name of Depositor
G Kohler, C Milstein
Deposited As
Mus musculus
References

Barnstable CJ, et al. Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens -- new tools for genetic analysis. Cell 14: 9-20, 1978. PubMed: 667938

Kohler G, et al. Fusion between immunoglobulin-secreting and nonsecreting myeloma cell lines. Eur. J. Immunol. 6: 292-295, 1976. PubMed: 825374

Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579

Kohler G, Milstein C. Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion. Eur. J. Immunol. 6: 511-519, 1976. PubMed: 825377

Sato JD, et al. Effects of proximate cholesterol precursors and steroid hormones on mouse myeloma growth in serum-free medium. In Vitro Cell. Dev. Biol. 24: 1223-1228, 1988. PubMed: 3209588

Ramasamy R, et al. Possible role for the Fc receptor on B lymphocytes. Nature 249: 573-574, 1974. PubMed: 4545851

Cowan NJ, et al. Intracellular immunoglobulin chain synthesis in non-secreting variants of a mouse myeloma: detection of inactive light-chain messenger RNA. J. Mol. Biol. 90: 691-701, 1974. PubMed: 4449137

Condie R, et al. Cannabinoid inhibition of adenylate cyclase-mediated signal transduction and interleukin 2 (IL-2) expression in the murine T-cell line, EL4.IL-2. J. Biol. Chem. 271: 13175-13183, 1996. PubMed: 8662742

Cross References

Nucleotide (GenBank) : AF248032 Mus musculus coatomer protein alpha subunit precursor, mRNA, partial cds.

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