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STC-1
STC-1
規格:
價格:
編號:B167196
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
STC-1
商品貨號
B167196
Organism
Mus musculus, mouse
Tissue
intestine
培養基
Cell Type
intestinal neuroendocrine tumor cells
Product Format
frozen 1.0 mL
Morphology
epithelial-like
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
invasive small intestinal neuroendocrine carcinoma
Age
10 to 13 weeks
Strain
C57B1/6J
Applications

This cell line may be a useful model for human neuroendocrine neoplasms of the gut, and useful tools for studying hormone secretin.

Storage Conditions
liquid nitrogen vapor phase
Images
Derivation
The STC-1 cell line was derived from the intestinal tumors of RIP1Tag2/Rip2pyST1 double transgenic mice.
Cellular Products
secretin
Comments
The STC-1 cell line was derived from the intestinal tumors of double transgenic mice. Transgenic mice harboring a hybrid gene linking the rat insulin promoter (RIP) to polyoma small T (PyST) antigen were mated with transgenic mice harboring rat insulin promoter (RIP) linked to SV40 early region (Tag) creating off-spring harboring both transgenes (double transgenics). These mice were found to have frequent intestinal tumors in addition to pancreatic Beta-cell tumors. Gene expression studies suggested that the intestinal and pancreatic tumors arose as separate entities. The STC-1cell line produces the hormone secretin. This cell line may be a useful model for human endocrine neoplasms of the gut.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cells must be subcultured when they reach ~70% confluence, or else they start to come off the flask into suspension.
Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 
    1. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) (ATCC 30-2200) or 0.05% Trypsin – 0.02% EDTA (ATCC PCS-999-003) solution to remove all traces of serum which contains trypsin inhibitor. 
    2. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). 
      Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. 
    3. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. 
    4. Transfer cell suspension to a centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes. 
    5. Discard supernatant. Resuspend the cell pellet in fresh growth medium. 
    6. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
Subcultivation Ratio: 1:3 to 1:5 is recommended.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C 
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Cells per Vial
1.0 x 10^6
Volume
1.0 mL
Name of Depositor
Douglas Hanahan, PhD Cold Spring Harbor Laboratory
Year of Origin
June 1990
References

Rindi G, et al. Development of Neuroendocrine Tumors in the Gastrointestinal Tract of Transgenic Mice. Am J Pathol 136(6): 1349-1363, 1990. PubMed: 2162628

Grant SGN, et al. Early Invasiveness Characterizes Metastatic Carcinoid Tumors in Transgenic Mice. Cancer Res 51: 4917-4923, 1991. PubMed: 1654206

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