最新中文字幕-精品国产99-国产精品福利在线观看-粉嫩av一区二区三区-日韩精品久久久久-美丽的姑娘国语视频-亚洲欧洲色-国产不卡毛片-三级黄毛片-免费福利在线-日韩av三区-欧美成年人网站-欧洲美女与动交ccoo-成人涩涩软件-秋霞影院午夜老牛影院-婷婷久久伊人-呦呦视频在线观看-www.天天综合-日韩精品高清视频-国产欧美综合在线-欧美日韩一区二区三区在线视频-a猛片免费播放-91九色蝌蚪国产-国产欧美日韩专区-嫩模被强到高潮呻吟不断

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > SU-DHL-2 B167179
SU-DHL-2
SU-DHL-2
規(guī)格:
價格:
編號:B167179
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
SU-DHL-2
商品貨號
B167179
Organism
Homo sapiens, human
Tissue
lymph node
Derived from Metastatic Site: peritoneal effusion
培養(yǎng)基
Cell Type
histiocytic
Product Format
frozen
Morphology
Lymphoblast-like
Culture Properties
suspension, multicell aggregates
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
large cell lymphoma; diffuse histiocytic lymphoma
Age
73 years
Gender
Female
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor phase
Karyotype
Most of these cells were hyperdiploid, with a sharp modal number of 51 chromosomes; occasional cells in the hypertetraploid range were also seen. A minute marker chromosome was consistently observed, and increased numbers of chromosomes were seen in the A, B, C, and F groups.
Images
CRL-2956 Cell Micrograph
Clinical Data
73 years
Caucasian
Female
Comments
The cells are non-specific esterase and acid phosphatase positive.
The cells are negative for Epstein-Barr virus nuclear antigen (EBNA-).
The cells are surface Ig negative (sIG-).
The cells are reported to be E - rosette negative.
ATCC confirmed this cell line is positive for the presence of Epstein-Barr viral DNA sequences via PCR.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Cultures can be maintained by the addition of fresh medium. An inoculum of 1 X 105 to 3 x 105 cells/mL is recommended. Subculture when cell concentration is between 7 x 105 and 1 x 106 cells/mL.
Subcultivation ratio: A subcultivation ratio of 1:3 to 1:8 is recommended.
Medium renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation

Storage temperature: liquid nitrogen vapor phase

Freeze medium: complete growth medium supplemented with an additional 20% fetal bovine serum and 10% (v/v) DMSO
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile

D5S818: 13
D13S317: 11, 12
D7S820: 11, 13
D16S539: 14
vWA: 17
THO1: 6, 9
TPOX: 8
CSF1PO: 12
Amelogenin: X
Name of Depositor
A Epstein
Year of Origin
1974
References

Epstein AL, et al. Biology of the human malignant lymphomas. IV. Functional characterization of ten diffuse histiocytic lymphoma cell lines. Cancer. 42(5): 2379-2391, 1978. PubMed: 214220

Siminovitch KA, et al. Immunoglobulin gene rearrangements and expression in diffuse histiocytic lymphomas reveal cellular lineage, molecular defects, and sites of chromosomal translocation. Blood. 67(2): 391-397, 1986. PubMed: 3080039

Epstein AL, Kaplan HS. Feeder layer and nutritional requirements for the establishment and cloning of human malignant lymphoma cell lines. Cancer Res. 39(5):1748-1759, 1979. PubMed: 371794

  • 王經(jīng)理
    手機(jī)號：17280875617
    QQ：1438578920
  • 胡經(jīng)理
    手機(jī)號：13345964880
    QQ：2438244627
  • 周經(jīng)理
    手機(jī)號：17757487661
    QQ：1296385441
  • 于經(jīng)理
    手機(jī)號：18067160830
    QQ：2088210172
  • 沈經(jīng)理
    手機(jī)號：19548299266
    QQ：2662369050
  • 李經(jīng)理
    手機(jī)號：13626845108
    QQ：972239479