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ZF4
ZF4
規格:
價格:
編號:B165992
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
ZF4
商品貨號
B165992
Organism
Danio rerio, zebrafish
Tissue
embryo
培養基
Product Format
frozen
Morphology
fibroblast
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age
1 day old embryo
Applications
The cells can be used as a transfection host.
Storage Conditions
liquid nitrogen vapor phase
Karyotype
hyperploid; range = 110 to 120
Images
Derivation
The ZF4 cell line was established from 1-day-old zebrafish embryos.
Comments

The ZF4 cell line was established from 1-day-old zebrafish embryos.

The cells are cultivated at 28°C.
ZF4 cells are killed within 10 days by culturing in medium containing 0.8 to 1.0 mg/mL G418.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 28°C.

NOTE: DO NOT USE EDTA TO DISSOCIATE THE CELLS.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 28°C
Name of Depositor
W Driever
Deposited As
Brachydanio rerio
References

Driever W, Rangini Z. Characterization of a cell line derived from zebrafish (Brachydanio rerio) embryos. In Vitro Cell. Dev. Biol. Anim. 29A: 749-754, 1993. PubMed: 8407719

Biosafety in Microbiological and Biomedical Laboratories 4th ed.; U.S. Department of Health and Human Services ;1999.

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