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YN1/1.7.4
YN1/1.7.4
規(guī)格:
價(jià)格:
編號(hào):B165989
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
YN1/1.7.4
商品貨號(hào)
B165989
Organism
Rattus norvegicus (B cell); Rattus norvegicus (myeloma), rat (B cell); rat (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with a 95000 to 100000 dalton antigen (ICAM-1) on activated and proliferating murine lymphocytes.
Derivation
Spleen cells were fused with Y3 rat myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against murine ICAM-1 (ICAM 1, intercellular adhesion molecule)
Cellular Products
immunoglobulin; monoclonal antibody; against murine ICAM-1 (ICAM 1, intercellular adhesion molecule)
Comments
Animals were immunized with NS-1 mouse myeloma cells.
Spleen cells were fused with Y3 rat myeloma cells.
The antibody reacts with a 95000 to 100000 dalton antigen (ICAM-1) on activated and proliferating murine lymphocytes.
The antibody partially inhibits Con A stimulation of spleen cells but does not inhibit LPS stimulation.
The antibody strongly inhibits mixed lymphocyte responses (MLR).
The ICAM-1 antigen is absent on most unstimulated thymocytes and spleen cells.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/ml and maintain between 1 X 105 and 1 X 106 cells/ml.
Isotype
IgG2b
Name of Depositor
F Takei
Deposited As
rat (B cell); rat (myeloma)
References

Takei F. Inhibition of mixed lymphocyte response by a rat monoclonal antibody to a novel murine lymphocyte activation antigen (MALA-2). J. Immunol. 134: 1403-1407, 1985. PubMed: 2981916

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