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Y-3
Y-3
規格:
價格:
編號:B165982
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Y-3
商品貨號
B165982
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody cross-reacts with the k, p, q, r and s H-2 haplotypes.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against H-2 K b
Cellular Products
immunoglobulin; monoclonal antibody; against H-2 K b
Comments
Animals were immunized with Con A stimulated spleen cells from BALB.B mice.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody cross-reacts with the k, p, q, r and s H-2 haplotypes.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG2b; kappa light chain
Name of Depositor
C Janeway
Deposited As
mouse (B cell); mouse (myeloma)
References

Hammerling GJ, et al. Localization of allodeterminants on H-2Kb antigens determined with monoclonal antibodies and H-2 mutant mice. Proc. Natl. Acad. Sci. USA 79: 4737-4741, 1982. PubMed: 6181513

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