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X16C8.5 [X16C]
X16C8.5 [X16C]
規格:
價格:
編號:B165964
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
X16C8.5 [X16C]
商品貨號
B165964
Organism
Mus musculus, mouse
Cell Type
B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
reticulum cell sarcoma
Strain
BALB/cAnN
Applications
Derived from a spontaneous reticulum cell neoplasm found in an old BALB/cAnN mouse.
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions
liquid nitrogen vapor phase
Karyotype
near tetraploid; modal number = 78; range = 39 to 84
Derivation
Derived from a spontaneous reticulum cell neoplasm found in an old BALB/cAnN mouse.
Antigen Expression
I-A+; H-2d
Receptor Expression
Fc
Genes Expressed
immunoglobulin (both surface and secreted, sIg+),I-A+; H-2d,Expression of I-A and H-2 is increased by treatment with interferon.
Cellular Products
immunoglobulin (both surface and secreted, sIg+)
Tumorigenic
No
Effects
No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.
Comments
Derived from a spontaneous reticulum cell neoplasm found in an old BALB/cAnN mouse.
Expression of I-A and H-2 is increased by treatment with interferon.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Isotype
IgM
Population Doubling Time
18 hrs
Name of Depositor
KJ Kim
Deposited As
Mus musculus
References

Kim KJ, et al. Establishment and characterization of BALB/c lymphoma lines with B cell properties. J. Immunol. 122: 549-554, 1979. PubMed: 310843

Kim KJ, et al. Characterization of T-cell-soluble factors modulating the expression of Ia and H-2 antigens on BALB/c B lymphoma cell lines. Cell. Immunol. 76: 253-267, 1983. PubMed: 6404559

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