Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
NOTE: Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination
EMEM (30-2003) supplemented with 10% FBS (30-2020)
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended
Medium Renewal: Twice per week
Hayflick L. The establishment of a line (WISH) of human amnion cells in continuous cultivation. Exp. Cell Res. 23: 14-20, 1961. PubMed: 13712490
Buynak EB, et al. Infectivity Stability of Live Measles-Virus Vaccine. Am. J. Dis. Child. 103:460, 1962.
Hulkower KI, et al. Induction of prostaglandin H synthase-2 and tumor necrosis factor-alpha in human amnionic WISH cells by various stimuli occurs through distinct intracellular mechanisms. J. Pharmacol. Exp. Ther. 280: 1065-1074, 1997. PubMed: 9023325
Lundgren DW, et al. Hypotonic stress increases cyclooxygenase-2 expression and prostaglandin release from amnion-derived WISH cells. J. Biol. Chem. 272: 20118-20124, 1997. PubMed: 9242685
Perkins DJ, Kniss DA. Rapid and transient induction of cyclo-oxygenase 2 by epidermal growth factor in human amnion-derived WISH cells. Biochem. J. 321: 677-681, 1997. PubMed: 9032453
