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Wgd5
Wgd5
規(guī)格:
價格:
編號:B165946
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Wgd5
商品貨號
B165946
Organism
Mus musculus, mouse
Tissue
connective tissue
培養(yǎng)基
Cell Type
fibroblast
Product Format
frozen
Morphology
fibroblast
Culture Properties
adherent
Biosafety Level
2 Cells contain polyomavirus DNA sequences

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Leukemia
Applications
transfection host
Helper cell line for packaging retroviral vectors such as those released from lambdaZd31, lambdaZd32, and lambdaZd35 (ATCC 37563-5, respectively).
Derivation
The line was derived from the WOP cell line (a 3T3 derivative transformed by an origin defective polyomavirus).
Genes Expressed
retrovirus vector (Moloney murine leukemia virus
Cellular Products
retrovirus vector (Moloney murine leukemia virus
Comments
The Wgd5 cell line contains a mutant of the Moloney murine leukemia virus (MoMuLV) that is useful for packaging recombinant retroviral DNA into infectious virus particles.
The line was derived from the WOP cell line (a 3T3 derivative transformed by an origin defective polyomavirus).
Wgd5 was selected as a gpt+ clone after cotransformation of WOP with pSV2gpt and a packaging mutant of MoMuLV.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Dulbecco's modified Eagle's medium with 0.25 mg/ml xanthine, 0.015 mg/ml hypoxanthine, 0.01 mg/ml thymidine, 0.002 mg/ml aminopterin and 0.015 mg/ml mycophenolic acid, 90%; fetal bovine serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution for 1 to 2 minutes, rinse and remove trypsin.
Allow the cells to sit at 37C until they begin to detach (about 3 to 5 minutes), add fresh medium, aspirate and dispense into new flasks.
Subculture before the cells become confluent.
If allowed to become confluent, the cells will slough off and die.
Name of Depositor
AJ Murphy
Deposited As
Mus musculus
References

Murphy AJ, Efstratiadis A. Cloning vectors for expression of cDNA libraries in mammalian cells. Proc. Natl. Acad. Sci. USA 84: 8277-8281, 1987. PubMed: 3479791

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