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WEHI-231
WEHI-231
規(guī)格:
價格:
編號:B165940
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
WEHI-231
商品貨號
B165940
Organism
Mus musculus, mouse
Cell Type
B lymphocyte, immature
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension, multicell aggregates
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
B cell lymphoma
Strain
(BALB/c x NZB)F1
Applications
This cell line is a suitable transfection host.
Storage Conditions
liquid nitrogen vapor phase
Images
Derivation
Mineral oil induced tumor in (BALB/c x NZB)F1 mice
Genes Expressed
immunoglobulin
Cellular Products
immunoglobulin
Comments
WEHI-231 cells do not secrete IgM into medium unless stimulated with lipopolysaccharide (0.01 to 3 μg/mL). Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.05 mM; fetal bovine serum to a final concentration of 10%.
Subculturing
Optimum culture recovery from a frozen vial is in a T25 flask at a density between 2 to 5 X 105 viable cells/mL. If recovered at 5 X 105 viable cells/mL, media will need to be added to the culture within the first 3 days, before the density reaches 1 X 106 viable cells/mL, at which point the viability drops drastically.
After cells recover, expansions can be done at 1 X 105 viable cells/mL by adding media to decrease the cell concentration.
This cell line forms tight clusters of viable cells accompanied by some single non-viable cells plus debris in suspension. With increased growth the clusters become larger. As the cell density nears 1 X 106 cells/mL, the single (non-clustered) and non-viable cells increase along with the amount of debris.
At subculture, break up clusters by gentle pipetting.
Established cultures can be grown in T75 flasks.
Subcultivation Ratio: Add fresh medium every 2 to 3 days (depending on cell density)
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Isotype
IgM , IgM (surface)
Population Doubling Time
about 20 hours
Name of Depositor
NL Warner, LL Lanier
Deposited As
Mus musculus
References

Boyd AW, et al. The regulation of growth and differentiation of a murine B cell lymphoma. I. Lipopolysaccharide induced differentiation. J. Immunol. 126: 2461-2465, 1981. PubMed: 6785355

Lanier LL, Warner NL. Cell cycle related heterogeneity of Ia antigen expression on a murine B lymphoma cell line:analysis by flow cytometry. J. Immunol. 126: 626-631, 1981. PubMed: 6969756

Gutman GA, et al. Immunoglobulin production by murine B-lymphoma cells. Clin. Immunol. Immunopathol. 18: 230-244, 1981. PubMed: 6781803

mineral oil induced tumor in (BALB/c x NZB)F1 mice

To secrete IgM into the medium, these cells need to be stimulated with lipopolysaccharide (0.01 to 3 mcg/ml).

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