最新中文字幕-精品国产99-国产精品福利在线观看-粉嫩av一区二区三区-日韩精品久久久久-美丽的姑娘国语视频-亚洲欧洲色-国产不卡毛片-三级黄毛片-免费福利在线-日韩av三区-欧美成年人网站-欧洲美女与动交ccoo-成人涩涩软件-秋霞影院午夜老牛影院-婷婷久久伊人-呦呦视频在线观看-www.天天综合-日韩精品高清视频-国产欧美综合在线-欧美日韩一区二区三区在线视频-a猛片免费播放-91九色蝌蚪国产-国产欧美日韩专区-嫩模被强到高潮呻吟不断

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車 1 種商品 - 共0元
當(dāng)前位置: 首頁(yè) > ATCC代理 > W6/32 B165933
W6/32
W6/32
規(guī)格:
價(jià)格:
編號(hào):B165933
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
W6/32
商品貨號(hào)
B165933
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Tissue
Spleen, tonsils
培養(yǎng)基
Cell Type
Hybridoma: B lymphocyte
Product Format
frozen
Morphology
Lymphoblast-like
Culture Properties
Suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain
BALB/c (B cell); BALB/c (myeloma)
Storage Conditions
liquid nitrogen vapor phase
Images
Derivation
Animals were immunized with cells from human tonsils. Spleen cells were fused with P3X63Ag8 myeloma cells.
Cellular Products
immunoglobulin; monoclonal antibody; against HLA A, B, C
Comments
The antibody reacts with monomorphic determinants on HLA A,B,C molecules. The antibody is cytotoxic. The antibody cross-reacts with ape, monkey and cow cells. It does not react with rabbit cells. Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL. 

Medium Renewal: Every 2 to 3 days

Cryopreservation
95% FBS ; 5% DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: 5% CO2
Isotype
IgG2a
Antigenic Determinant
HLA-A, B, C
Name of Depositor
P Parham
Deposited As
mouse (B cell); mouse (myeloma)
References

Brodsky FM, Parham P. Monomorphic anti-HLA-A,B,C monoclonal antibodies detecting molecular subunits and combinatorial determinants. J. Immunol. 128: 129-135, 1982. PubMed: 6172474

Barnstable CJ, et al. Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens -- new tools for genetic analysis. Cell 14: 9-20, 1978. PubMed: 667938

Parham P, et al. Use of a monoclonal antibody (W6/32) in structural studies of HLA- A,B,C, antigens. J. Immunol. 123: 342-349, 1979. PubMed: 87477

Solomon KR, et al. The association between glycoslphosphatidylinositol-anchored proteins and hetrotrimeric G protein alpha subunits in lymphocytes. Proc. Natl. Acad. Sci. USA 93: 6053-6058, 1996. PubMed: 8650218

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

  • 王經(jīng)理
    手機(jī)號(hào):17280875617
    QQ:1438578920
  • 胡經(jīng)理
    手機(jī)號(hào):13345964880
    QQ:2438244627
  • 周經(jīng)理
    手機(jī)號(hào):17757487661
    QQ:1296385441
  • 于經(jīng)理
    手機(jī)號(hào):18067160830
    QQ:2088210172
  • 沈經(jīng)理
    手機(jī)號(hào):19548299266
    QQ:2662369050
  • 李經(jīng)理
    手機(jī)號(hào):13626845108
    QQ:972239479