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VERO 76
VERO 76
規格:
價格:
編號:B165924
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
VERO 76
商品貨號
B165924
Organism
Cercopithecus aethiops
Tissue
kidney
培養基
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Normal
Age
adult
Storage Conditions
liquid nitrogen vapor temperature
Derivation
VERO 76 was derived in 1968 by K.M. Johnson and brought to Centers for Disease Control (CDC), Atlanta, GA. It is a derivative of the original VERO.
Comments
When infected with hemorrhagic fever viruses [Machupo (Bolivian), Junin (Argentinian), Lassa (African)], Marburg or Ebola viruses, these cells exhibited cytopathic effects. Plaques are also produced. VERO 76 has a lower saturation density than the original VERO (ATCC CCL-81).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37°C
Name of Depositor
EM Earley
Deposited As
Cercopithecus aethiops
Year of Origin
1968
References

Earley EM, Johnson KMThe lineage of Vero, Vero 76 and its clone C1008 in the United StatesIn: Earley EM, Johnson KMVero cells: origin, properties and biomedical applicationsTokyoChiba Univ.pp. 26-29, 1988

Policastro PF, et al. Improved plaque assays for Rickettsia prowazekii in Vero76 cells. J. Clin. Microbiol. 34: 1944-1948, 1996. PubMed: 8818887

Schramm N, et al. Vesicles containing Chlamydia trachomatis serovar L2 remain above pH 6 within HEC-1B cells. Infect. Immun. 64: 1208-1214, 1996. PubMed: 8606080

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