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VD-10
VD-10
規格:
價格:
編號:B165922
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
VD-10
商品貨號
B165922
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with a 250000 dalton polypeptide that is part of the saxitoxin binding sodium channel protein in native electroplex membrane.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Animals were immunized with purified saxitoxin binding component of electric eel (E. electricus) electroplex membrane.
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against saxitoxin binding component of electroplex membrane
Cellular Products
immunoglobulin; monoclonal antibody; against saxitoxin binding component of electroplex membrane
Comments
Animals were immunized with purified saxitoxin binding component of electric eel (E. electricus) electroplex membrane.
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with a 250000 dalton polypeptide that is part of the saxitoxin binding sodium channel protein in native electroplex membrane.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
RPMI 1640 medium, 80%; fetal bovine serum, 20%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG1
Name of Depositor
LC Fritz, JP Brockes
Deposited As
mouse (B cell); mouse (myeloma)
References

Moore HP, et al. Isolation and characterization of a monoclonal antibody against the saxitoxin-binding component from the electric organ of the eel Electrophorus electricus. Proc. Natl. Acad. Sci. USA 79: 1673-1677, 1982. PubMed: 6280195

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