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USASK/DSIL-LHRH-A1

規格:

價格:

編號:B165914

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

USASK/DSIL-LHRH-A1

商品貨號

B165914

Organism

Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)

Cell Type

hybridoma: B lymphocyte

培養基

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications

It is recommended that the feeder cells be plated 24 hours before use at 2 X 10(6)/T75 in order to obtain a 30% confluent monolayer.

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Derivation

Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.

Genes Expressed

immunoglobulin; monoclonal antibody; against the carboxy terminal end of gonadotropin releasing hormone (also called luteinizing hormone releasing hormone (LHRH)

Cellular Products

immunoglobulin; monoclonal antibody; against the carboxy terminal end of gonadotropin releasing hormone (also called luteinizing hormone releasing hormone (LHRH)

Tumorigenic

Yes

Effects

Yes, forms ascites in pristane primed BALB/c mice

Comments

Animals were immunized with LHRH conjugated to bovine serum albumin.

Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.

A feeder layer is required to recover the cells from a frozen ampule. Cells are grown at ATCC on 56-X feeder layer, irradiated STO cells. It is recommended that the feeder cells be plated 24 hours before use at 2 X 10(6)/T75 in order to obtain a 30% confluent monolayer.

Complete Growth Medium

RPMI 1640 medium with 2 mM L-glutamine and 50 mg/l gentamicin, 70%; NCTC 109 medium, 10%; fetal bovine serum, 20%

Subculturing

Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. Cells are grown at ATCC on 56-X feeder layer, irradiated STO cells. It is recommended that the feeder cells be plated 24 hours before use at 2 X 10(6)/T75 in order to obtain a 30% confluent monolayer.

Cryopreservation

Culture medium, 95%; DMSO, 5%

Isotype

IgG1

Name of Depositor

University of Saskatchewan

Deposited As

mouse (B cell); mouse (myeloma)

U.S. Patent Number

4,676,981

References

Silversides DW, et al. Monoclonal antibodies against LHRH: development and immunoactivity in vivo and in vitro. J. Reprod. Immunol. 7: 171-184, 1985. PubMed: 3884802

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