Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
The antibody is useful as a control monoclonal antibody when using other hamster derived hybridomas.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is useful as a control monoclonal antibody when using other hamster derived hybridomas.
Genes Expressed
immunoglobulin; monoclonal antibody; against dinitrophenyl (DNP)
Cellular Products
immunoglobulin; monoclonal antibody; against dinitrophenyl (DNP)
Comments
Animals were immunized with DNP derivatized KLH (keyhole limpet hemocyanin).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is useful as a control monoclonal antibody when using other hamster derived hybridomas.
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
