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tsc2 ang1
tsc2 ang1
規格:
價格:
編號:B165887
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
tsc2 ang1
商品貨號
B165887
Organism
Mus musculus, mouse
Product Format
frozen
培養基
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
cutaneous sarcoma
Applications
The tsc2 ang1 cell line was derived in 1998 from a cutaneous sarcoma taken from a Tsc2+/- mouse heterozygous for tuberin.
It can be used for drug testing and for angiogenesis studies.
The cell line is a useful model of TSC. It is the only genetically defined tuberous sclerosis cell line available that forms tumors.
Derivation
Tuberous sclerosis (TSC) is an autosomal dominant genetic disorder caused by mutations in either TSC1 or TSC2. Mice heterozygous for Tsc2 were developed as a murine model of Tsc2 disease using a gene targeting approach.
The tsc2 ang1 cell line was derived in 1998 from a cutaneous sarcoma taken from a Tsc2+/- mouse heterozygous for tuberin.
Tumorigenic
Yes
Effects
Yes, forms tumors in nude mice
Comments
Tuberous sclerosis (TSC) is an autosomal dominant genetic disorder caused by mutations in either TSC1 or TSC2. Mice heterozygous for Tsc2 were developed as a murine model of Tsc2 disease using a gene targeting approach.
The tsc2 ang1 cell line was derived in 1998 from a cutaneous sarcoma taken from a Tsc2+/- mouse heterozygous for tuberin.
The cell line is a useful model of TSC. It is the only genetically defined tuberous sclerosis cell line available that forms tumors. It can be used for drug testing and for angiogenesis studies.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:5 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor
JL Arbiser
Deposited As
mouse
Year of Origin
1998
References

Onda H, et al. Tsc2(+/-) mice develop tumors in multiple sites that express gelsolin and are influenced by genetic background. J. Clin. Invest. 104: 687-695, 1999. PubMed: 10491404

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