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TOV-112D

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價格:

編號:B165875

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

TOV-112D

商品貨號

B165875

Organism

Homo sapiens, human

Tissue

Ovary

培養基

Product Format

frozen

Morphology

epithelial

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

grade 3, stage IIIC, primary malignant adenocarcinoma; endometrioid carcinoma

Age

42 years adult

Gender

female

Storage Conditions

liquid nitrogen vapor temperature

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Karyotype

52, XX, add(X)(p22), +add(1)(p22), +add(1)(p22), +2, +9, +12, add(15)(p11), +17 Ref

Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993

Images

Derivation

This cell line was initiated in October of 1992 from a patient with early onset ovarian cancer. The patient was of French-Canadian descent with an unknown family history of ovarian cancer.

Clinical Data

female

This cell line was initiated in October of 1992 from a patient with early onset ovarian cancer.

Oncogene

her2/neu +, p53 (mutated, Arg --> His mutation at exon 6, codon 175) Ref

Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993

Genes Expressed

keratin

Cellular Products

keratin

Tumorigenic

Yes

Effects

The cells are tumorigenic in nude mice and form colonies in soft agar

Comments

Unlike TOV-21G (ATCC CRL-11730) and OV-90 (ATCC CRL-11732), the TOV-112D cell line does not exhibit a deletion at chromosome 3p24.

Complete Growth Medium

The base medium for this cell line is a 1:1 mixture of MCDB 105 medium containing a final concentration of 1.5 g/L sodium bicarbonate and Medium 199 containing a final concentration of 2.2 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium:

fetal bovine serum to a final concentration of 15%

Subculturing

Remove medium, add fresh 0.05% trypsin - 0.53 mM EDTA, rinse and remove trypsin. Allow the culture sit at room temperature (or 37°C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Medium Renewal: Every 3 to 4 days

Cryopreservation

Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Culture Conditions

Temperature: 37°C

STR Profile

Amelogenin: X

CSF1PO: 12

D13S317: 8

D16S539: 9,12

D5S818: 10

D7S820: 9,10

THO1: 6

TPOX: 8,11

vWA: 18

Name of Depositor

University of Montreal

Deposited As

human

U.S. Patent Number

5,710,038

Year of Origin

1992

References

Mes-Masson AM, Provencher D. Primary cultures of normal and tumoral human ovarian epithelium. US Patent 5,710,038 dated Jan 20 1998

Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993

B228282：Human genomic DNA (TOV-112D)

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