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TGP47
TGP47
規格:
價格:
編號:B165854
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
TGP47
商品貨號
B165854
Organism
Mus musculus, transgenic, mouse, transgenic
Tissue
pancreas
培養基
Cell Type
Epithelial
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
2 CELLS CONTAIN PAPOVAVIRUS

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
pancreatic acinar cell tumor; carcinoma
Age
26 weeks
Gender
male
Strain
Tg(E1a-1-SV40E)Bri18
Applications
TGP47 is an epithelial like cell line derived from a pancreatic tumor with acinar cell morphology that arose in an adult male transgenic mouse.
The mouse carried the pseudogene construct composed of elastase-1 promoter linked to the SV40 T antigen.
The cells do not secrete amylase or lipase.
Histochemical staining of the current stock of TGP47 was negative for somatostatin and positive for trypsin.
Derivation
TGP47 is an epithelial like cell line derived from a pancreatic tumor with acinar cell morphology that arose in an adult male transgenic mouse.
Clinical Data
TGP47 is an epithelial like cell line derived from a pancreatic tumor with acinar cell morphology that arose in an adult male transgenic mouse.
male
Comments
TGP47 is an epithelial like cell line derived from a pancreatic tumor with acinar cell morphology that arose in an adult male transgenic mouse.
The mouse carried the pseudogene construct composed of elastase-1 promoter linked to the SV40 T antigen.
TGP47 cells appear somewhat duct like in culture, but contain rare somatostatin positive cells.
The cells do not secrete amylase or lipase.
Histochemical staining of the current stock of TGP47 was negative for somatostatin and positive for trypsin.
Morphologic and differentiation markers of this line appear to fluctuate from time to time in culture.
Complete Growth Medium
A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium, 90%; heat-inactivated fetal bovine serum, 10%
Subculturing
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Twice per week
Cryopreservation
Culture medium, 95%; DMSO, 5%
Name of Depositor
OS Pettengill, D Longnecker
Deposited As
mouse, transgenic
References

Pettengill OS, et al. Cell lines derived from pancreatic tumors of Tg(Ela-1-SV40E)Bri18 transgenic mice express somatostatin and T antigen. Carcinogenesis 15: 61-65, 1994. PubMed: 7904904

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