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Tera-1
Tera-1
規(guī)格:
價格:
編號:B165850
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱
Tera-1
商品貨號
B165850
Organism
Homo sapiens, human
Tissue
testes; derived from metastatic site: lung
培養(yǎng)基
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
embryonal carcinoma, malignant
Age
47 years
Gender
male
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor phase
Karyotype
at passage 20, hypotriploid (+A, +A3, +B, +C, +D, +E, +F, -A2) with abnormalities including endoreduplications, acrocentric fragments, minutes, translocations, secondary constrictions, breaks and large metacentric markers
Clinical Data
47 years adult
Caucasian
male
Tumorigenic
No
Effects
No, in immunosuppressed mice
Yes, in semisolid medium
Complete Growth Medium
The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified , Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
Subculturing
Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells from the floor of the flask, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Subculture every 6 to 8 days.
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 11
D13S317: 12
D16S539: 10,12
D5S818: 11,13
D7S820: 9,10
THO1: 7,8
TPOX: 8
vWA: 14
Isoenzymes
AK-1, 1
ES-D, 2
G6PD, B
GLO-I, 1-2
Me-2, 1-2
PGM1, 1
PGM3, 1-2
Name of Depositor
J Fogh
Deposited As
Homo sapiens
References

Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

Faust JB, Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. PubMed: 1568216

Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047

Schar BK, et al. Simultaneous detection of all four alkaline phosphatase isoenzymes in human germ cell tumors using reverse transcription-PCR. Cancer Res. 57: 3841-3846, 1997. PubMed: 9288797

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