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TE7
TE7
規格:
價格:
編號:B165847
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
TE7
商品貨號
B165847
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with cells in the thymic capsule and interlobular septae and vessels.
It also reacts with non-endocrine fibrous stroma in the thymus, and with mesodermally derived connective tissue in other parts of the body.
It defines the mesodermal component of the human thymic microenvironment that most likely induces thymic epithelial differentiation early in the development of the thymus.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
It also reacts with non-endocrine fibrous stroma in the thymus, and with mesodermally derived connective tissue in other parts of the body.
Genes Expressed
immunoglobulin; monoclonal antibody; against human thymus
Cellular Products
immunoglobulin; monoclonal antibody; against human thymus
Comments
Animals were immunized with human thymic stroma.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with cells in the thymic capsule and interlobular septae and vessels.
It also reacts with non-endocrine fibrous stroma in the thymus, and with mesodermally derived connective tissue in other parts of the body.
It defines the mesodermal component of the human thymic microenvironment that most likely induces thymic epithelial differentiation early in the development of the thymus.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Culture Medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 80%;
fetal bovine serum, 20%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG1
Name of Depositor
BF Haynes
Deposited As
mouse (B cell); mouse (myeloma)
References

Haynes BF, et al. Phenotypic characterization and ontogeny of mesodermal-derived and endocrine epithelial components of the human thymic microenvironment. J. Exp. Med. 159: 1149-1168, 1984. PubMed: 6200562

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