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TBM-54

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價格:

編號:B165829

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

TBM-54

商品貨號

B165829

Organism

Bufo marinus, toad, tropical

Tissue

urinary bladder

培養基

Cell Type

Epithelial

Product Format

frozen

Morphology

epithelial

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications

TBM-54 is an epithelial cell line derived in 1977 from the urinary bladder of a tropical toad collected in the Dominican Republic.

The cells resemble the granular cell type of the urinary bladder.

These cells form an epithelium with typical tight junctions and gap junctions and they form multilayers with one layer of undifferentiated cells interposed between the apical layer and the supporting structure.

They exhibit hormone sensitive transepithelial electrical resistance, actively transport sodium and are subject to hormonal control in culture.

TBM-54 cells do not respond to vasopressin.

Aldosterone increases sodium transport in a manner similar to that observed in intact bladder.

TBM-54 cells exhibit an excellent response to mineralocortioids in serum free medium.

Derivation

TBM-54 is an epithelial cell line derived in 1977 from the urinary bladder of a tropical toad collected in the Dominican Republic.

Comments

TBM-54 is an epithelial cell line derived in 1977 from the urinary bladder of a tropical toad collected in the Dominican Republic.

The cells resemble the granular cell type of the urinary bladder.

They have dense granules near the apical surface characteristic of the granular cell type found in the intact bladder.

They exhibit hormone sensitive transepithelial electrical resistance, actively transport sodium and are subject to hormonal control in culture.

Development of transepithelial electrical resistance (R) and transport rate (Isc) are dependent on time and the density of cells seeded, but steady state Isc and R is characteristic of the cell line and independent of seeding density.

TBM-54 cells do not respond to vasopressin.

Analogues of cAMP increase sodium transport and urea permeability but do not effect water permeability.

Aldosterone increases sodium transport in a manner similar to that observed in intact bladder.

TBM-54 cells exhibit an excellent response to mineralocortioids in serum free medium.

Complete Growth Medium

A 7:3 mixture of Coon's F-12 and Leibovitz's L-15 medium modified to contain 103 mM NaCl and 25 mM NaHCO3 with 1 nM dexamethasone, 30 nM spermine, 250 nM spermidine, 130 nM putrescine, 0.01 mg/ml insulin, 95%; heat-inactivated fetal bovine serum, 5%

Subculturing

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended

Medium Renewal: 3 times per week

Remove medium, and rinse the monolayer with fresh 0.25% trypsin solution. Remove the trypsin solution, and let the culture sit at room temperature until the cells detach (about 5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks.

Cryopreservation

Culture medium, 95%; DMSO, 5%

Name of Depositor

J Handler, B Rossier

Deposited As

Bufo marinus

Year of Origin

1977

References

Handler JS, et al. Toad urinary bladder epithelial cells in culture: maintenance of epithelial structure, sodium transport, and response to hormones. Proc. Natl. Acad. Sci. USA 76: 4151-4155, 1979. PubMed: 226998

Johnson JP, et al. Epithelial organization and hormone sensitivity of toad urinary bladder cells in culture. Am. J. Physiol. 241: F129-F138, 1981. PubMed: 6267945

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